Device
miniToe po

Part:BBa_K2615018:Design

Designed by: Yunqian Zhang   Group: iGEM18_OUC-China   (2018-09-10)


MiniToe polycistron A: a new system using miniToe.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 197


Design Notes

We use miniToe-WT(BBa_K2615020) before and behind miniToe polycistron A(BBa_K2615018), meanwhile the miniToe-5(BBa_K2615025) is used between two CDS. And we use sfGFP and mCherry to test our system.

Source

Science Museum No.105，College of Marine Life Sciences, Ocean University of China

References